Six days after IR, cells were treated with either dimethyl sulfoxide (DMSO) or Baf A1 for 24 hours before sampling to block GATA4 and p62 degradation. ( G) Immunoblotting of Flag-GATA4 immunoprecipitates from proliferating (−IR) or IR-induced senescent IMR90 cells. ( E and F) IMR90 cells were transfected with the indicated siRNAs, and proteins were analyzed by Western blotting 79 hours after transfection cont denotes firefly luciferase siRNA. Data are mean ± SEM one-way ANOVA was used for the statistical analysis. Shown are representative immunoblots (left) and quantification from four independent experiments (right, 24 hours of treatment). ( D) IMR90 cells were treated with the indicated autophagy inhibitory agents for the indicated times, and proteins were analyzed by immunoblotting with the indicated antibodies. p21 served as a positive control for MG-132. ( C) IMR90 cells were treated with the proteasomal inhibitor MG-132 for the indicated times, and proteins were analyzed by Western blotting. Shown are representative immunoblots (top) and quantification from three independent experiments (bottom). ( B) GATA4 protein stability was examined in the presence of cycloheximide (CHX) in proliferating (−IR) or IR-induced senescent cells. Relative abundance of GATA4 mRNA is expressed as change with respect to expression in cells without IR treatment. ( A) Western blot showing abundance of GATA4 protein during IR-induced senescence (top), oncogene (RAS V12)–induced senescence (middle left), or replicative senescence (middle right) abundance of GATA4 mRNA during IR-induced senescence is shown at the bottom. Data are representative of four (A) or three independent experiments.
Left: Population doubling analysis Right: Quantification of SA-β-Gal staining. ( E) Replicative senescence was assessed by cell growth analysis of BJ cells expressing either control or GATA4 shRNAs. Data are mean ± SEM one-way analysis of variance (ANOVA) was used for the statistical analysis. ( D) BJ cells carrying either a vector expressing a control shRNA targeting firefly luciferase or a GATA4 shRNA were exposed to IR (8 Gy), and 7 days later immunoblotting analysis (left) and SA-β-Gal staining (right) were performed. ( C) BJ cells carrying either a Dox-inducible (Tet-On) vector expressing GATA4 (Tet-GATA4) or an empty vector (Tet-Vector) were grown with or without Dox, and SA-β-Gal staining (left) and BrdU incorporation (right) were analyzed. Right: Immunoblotting analysis shows the efficiency of GATA4 depletion. Center: MFI was computed and normalized to control siRNA (−IR) data are mean ± SEM. ( B) Left: IMR90 cells expressing the miR-146a GFP reporter and transfected with the indicated siRNAs were exposed to IR (12 Gy), and GFP fluorescence was measured by FACS cont denotes the firefly luciferase siRNA control. Right: Median fluorescence intensity (MFI) was computed and normalized to vector control. Data are shown as percentage of maximum expression (i.e., the number of cells in each bin divided by the number of cells in the bin that contains the largest number of cells) for normalization. Published on Remmi Demmi Live Mix – Mr.( A) Left: IMR90 cells expressing the miR-146a GFP reporter were infected with either vector control or GATA4-expressing viruses, and GFP fluorescence was measured by fluorescence-activated cell sorter (FACS) (AFU, arbitrary fluorescence units). Published on Summerjam 2014 Showcase – Mr. Published on Ruhr Reggae Summer DJ Showcase 2015 – Video in description
#MARTIN JONDO 2015 FREE#
MRNICEGUY’s tracks Free mixes: Follow me at Hearthis.at/mrniceguy79 Nice Guy’s shows all share the same vibe: unheard sound meets a contagious smile behind the turntables. Nice Guy isn’t traveling around as a Genre-Bending DJ, he’s spinning Reggae Riddims as Martin Jondo’s Tour DJ at festivals like Summerjam or Ruhr Reggae Summer. Corporate gigs? How about Schalke 04 XMAS Party or G-Star Shoes HQ opening? Private parties? Kevin Prince Boateng and German National player Benedikt Höwedes have already booked him for private shows.
#MARTIN JONDO 2015 SERIES#
1 club moondoo made him the host of their weekly CMYKlub Friday series for over 4 months. His unique style of selection and mixing have earned him an exclusive invite to Red Bull Thre3Style and a Red Bull Most Wanted DJ title. He’s more likely to serve fresh mashups and unheard remixes in between the hits. Don’t expect him to just mess around with top 40 chart.
#MARTIN JONDO 2015 HOW TO#
Nice Guy knows how to stand out of the crowd. Having won multiple advertising awards, Mr.